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Fig. 5. (A) Domain structure of pp60Src and amino acid sequence of the SH2 domain (from mouse neuronal Src). (B) Schematic representation of the YFP-SH2 and YFP-dSH2 constructs. (C) Live-cell recording of SV80 cells transfected with YFP-SH2. The fusion protein localizes to FAs only weakly and shows a high cytoplasmic background. (D) Live-cell recording of SV80 cells transfected with YFP-dSH2. Note the distinct FA staining and relatively low cytoplasmic background. (E) Fixed cell transfected with YFP-SH2, showing loss of FA localization. (F) Fixed, YFP-dSH2 transfected cell, showing retention of FA labeling after fixation. Bar, 10 µm.





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