Fig. 9. (A) FRIT images (fluorescence ratio of images taken at different time points) of a movie showing an SV80 cell transfected with YFP-dSH2, serum starved, and treated with nocodazole for 30 minutes. The four spectrum scale images (see lookup table) are temporal ratios of two consecutive frames. Structures that appear only at the later image are shown in red, whereas structures that appear only at the earlier of the two time points are blue. Unchanged structures are represented in yellow. The numbers inserted into the 6'/2' image mark FAs that were further subjected to quantitative analysis (see below). The arrow points to a FA that is not present at 6' but appears at 10', whereas the arrowhead points to a FA that is present at 18' but has disappeared at 30'. The whole movie can be found in the online supplemental material (http://jcs.biologists.org/supplemental). Bar, 10 µm. (B) Quantitative analysis of the four FAs that were marked above (I-IV). The temporal changes of their average intensities and sizes are depicted. Often, neighboring FAs show different PY dynamics (compare FA I and II), and the changes in PY intensity are not always correlated in time with the growth of the FA. Note that the changes in PY level and FA size may be correlated in some cases (e.g. adhesions II and IV), or may be completely different (e.g. adhesions 1 and 3).

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