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Fig. 7. A subset of vacuoles induced by Gas3/PMP22 are coated with actin. NIH3T3 cells were microinjected with HA-Arf6-Q67L, with Gas3/PMP22-GFP or with PH-GFP and Gas3/PMP22-VSV. 15 hours later cells were fixed and probed with an antibody to HA and phalloidin (top), with phalloidin (middle), or with an antibody to VSV and phalloidin (bottom) as shown. (Inset) Enlargement of box showing differences in actin coating and PIP2 accumulation in Gas3/PMP22 vacuoles. Bar, 3 µm.





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