Fig. 2. Apical membrane reorganisation fails to occur in Df(1)MR mutants. Confocal images of wild-type and Df(1)MR pupal wings of different stages stained as indicated. Cartoons show cell outlines (grey) and the position of the apical junctions (black) in a transverse section of the epithelium at each stage. At 28-32 hours APF, wild-type and mutant epithelia are indistinguishable. By 46-50 hours APF, the formation of hair pedestals accumulating large amounts of actin (arrowheads) is hindered in the mutant. Note also the presence of an actin-ring around each cell in the mutant, visible also in the transverse sections (open arrowheads). The mutant cells also fail to acquire a star-like cell contour at the level of their apical junctions. At 62-66 hours APF, the rhodamin-phalloidin binds the apical membrane that appears folded inside the wing. Note that in the mutant a space between the apical membrane folds is present, where cuticle is secreted (open arrowheads). The aberrant apical junctions of the mutants also accumulate large amounts of -catenin—GFP. Bar, 5 µm.

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