Fig. 8. Double immunofluorescence micrographs showing the distribution of tyrosinated -tubulin (A,C,E,G,I) and ß_gc (B,D,F,H,J) in hippocampal pyramidal neurons after 24 hours (A,B,E,F) or 36 hours (C,D,G-J) in culture, in the absence (A-D) or the presence (E-J) of 5 ng ml^-1 BDNF. In the absence of BDNF, neurons exhibit faint ß_gc immunofluorescence in the growth cones (B and D, arrows). In the presence of BDNF, ß_gc immunofluorescence is strong in the growth cones and distal third of the axons (F and H, arrows). The addition of anti-sense KIF2 (asKIF2), even in the presence of BDNF (I and J, 36 hours in culture), greatly reduces axonal growth and abolishes distal ß_gc. Bar, 10 µm.

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