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Fig. 6. Mom-targeting assay. Dscs 1a, 2a and 3a in the mom flag vector were
contransfected with the p0071 head domain in PtK2 cells and visualized by
anti-flag antibody. Dsc3a and p0071 head colocalize on mitochondria, whereas
dsc1a and dsc2a localization is distinct from p0071 head distribution.
E-cadherin in the mom-vector with 4A6 tag was cotransfected with the p0071
head, repeat and tail domains. Whereas the p0071 repeat domain was recruited
to E-cadherin-coated mitochondria, head and tail domains of p0071 were not
recruited. Bars, 3 µm.