Fig. 3. Colonial morphology of P. marneffei cflB strains. (A) Colonial morphology of the P. marneffei cflB strain compared with the parental SPM4 control strain (cflB⁺). Strains were grown at 25°C for 14 days. The cflB colonies have a fluffy phenotype with reduced conidiation. At higher magnification (2x), no conidiophore structures were evident in the cflB strain. (B) Colonial morphology of P. marneffei cflB mutant transformants. Strains were grown for 12 days at 25°C on noninducing (Nonind.) or inducing medium (Induced). The alcA(p)::cflB^D123A dominant-negative transformants displayed reduced conidiation and an increase in the number of aerial hyphae under inducing conditions and the alcA(p)::cflB^G18V dominant-activated transformants displayed patchy conidiation that is more apparent when viewed under higher magnification (2x).

Return to article