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Fig. 6. Loss of cflB leads to an increase in apical branching in vegetative hyphal cells. Strains were grown for 2 days at 25°C (cflB+ and {Delta}cflB::pyrG+) or for 1 day and induced for an additional day [alcA(p)::cflBD123A and alcA(p)::cflBG18V]. Apical hyphal cells in the wild type are not branched. The {Delta}cflB and alcA(p)::cflBD123A dominant-negative strains showed hyperbranched apical hyphal cells. The alcA(p)::cflBG18V dominant-activated transformants possessed unbranched apical cells that displayed slightly irregular morphology. Scale bars, 20 µm. Images were captured using DIC or DAPI (to observe nuclei).





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