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Fig. 5. Ultrastructural localization of MAGI-3. Caco-2 cells were labeled with an antibody to MAGI-3 and a colloidal gold-conjugated secondary goat anti-rabbit antibody. (A) An immunoelectron micrograph showing labeling of the gold particles at tight junctional complex region (red arrows), nucleus (n; red arrowheads) and microvilli (mv: red asterisks) are presented. (B,C) Higher magnification images, showing the localization of MAGI-3 at tight junctions. Bar, A, 200 nm; B,C, 35 nm.





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