Fig. 4. Immunoisolation of SGs. (A) A PNS of PC12 cells was fractionated by differential centrifugation. The fraction enriched in SGs was incubated with myosin Va-specific serum (DIL2), control serum (cs) or without serum (w/o). The membranes bound to antibodies were isolated by immunoprecipitation with magnetic beads covalently coated with secondary antibodies. Bound (bound) and unbound membranes (unbound) were analysed by western blotting using antibodies against SgII. The signals for SgII (SgII) and the IgG heavy chain (IgG) are indicated. (B) The bar graph shows a quantitative analysis of seven independent experiments. For each experiment the sum of SgII in the respective unbound and bound fraction was set to 100%. Note that the differences between values obtained for the myosin Va serum and the controls are highly significant (paired t-test, P<0.005). Error bar, s.e.m.

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