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Fig. 4. Immunocytochemical analysis of E6-expressing AG05229 cells. (A-D) Cycling
E6-infected cells. (E-H) E6-infected cells at Mint. The assays are:
PC, phase contrast; p21, immunocytochemical detection of
p21Waf1; SAßgal, histochemical assessment of
SAß-gal activity; TUNEL, assay for apoptosis. Arrows indicate
multinucleate cells (F) and apoptotic cells (H). All cells except PC are
counterstained with haematoxylin. Cell-based assays were done in triplicate.
White bar, 100 µm (A,E); black bar, 100 µm (B-D,F-H).