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Fig. 2. Direct interaction of LPP and {alpha}-actinin in a blot overlay. Full-length LPP (right) as well as its N-terminal sequence (residues 1-109, left) were expressed in bacteria as GST-fusion proteins and resolved on polyacrylamide gels (0.1-2 µg/lane). After transfer to nitrocellulose, the proteins were probed with radiolabeled {alpha}-actinin. GST (2 µg/lane) and various protein standards were included as controls.





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