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Fig. 8. S1P amplifies the ephrinA1-mediated repression of VSMC spreading. Representative phase-contrast microscopy of quiescent VSMCs cultured for 16 hours in the adhesion assay with vehicle (a-d) or with 0.5 µM S1P (e-h). VSMCs where seeded on tissue culture dishes coated with laminin (0.25 µg/cm2) and either 1 µg/cm2 of Fc (a,e) or 0.2 (b,f), 0.5 (c,g), 1.0 (d,h) µg/cm2 of ephrinA1/Fc. Bar, 20 µm.





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