Fig. 8. A keratin particle moving towards the centrosome (Movie 8). PtK2 cells were transfected with EGFP-K5 N176S (green) and EYFP–tubulin (red). (A) The microtubule radial array and the centrosome, next to the nucleus of a transfected cell. Before imaging, cells were exposed to a heat shock to induce increased keratin particle formation. (B-E) A close-up view of the centrosome region 1 hour after the heat shock. A keratin particle (arrow) is clearly seen moving towards the centrosome and following microtubule tracks. Images from 21 time-points (see Movie 8) were collected (4 Z sections per time-point, 200 nm spacing). Panel images correspond to section 3, processed for edge enhancement. Bar, 1 µm.

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