Fig. 3. Effect of pH on esophageal epithelial cell growth and proliferation on a transwell filter device. Cells were grown by maintaining neutral pH into the basolateral chamber and adding acid pH or neutral pH into the apical chamber. (A) Cell number is given as the mean ± s.e.m. of three independent cultures. (B) Cell lysates of cells at D+1 were used for immunblotting using anti-PCNA antibody. Equal amount of protein per lane (10 µg) were analyzed.

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