Fig. 3. Fragmentation of mitochondria during partial irradiation with CMXRos. (A) Confocal images showing the irradiated part of a 143B cell stably expressing MtGFP (loaded with CMXRos) at various times during partial irradiation. The total duration of partial irradiation was 128 seconds. Change in mitochondrial morphology is representative of that in all 7 cells analysed. (B) Confocal images showing the irradiated part of a control cell stably expressing MtGFP that was partially irradiated without CMXRos under the same conditions as in A. Retention of filamentous morphology is representative of that in all five cells analysed. Gain levels were adjusted for images in both A and B to permit visualisation of mitochondria during intensive laser scanning, which results in some photobleaching of GFP and CMXRos. Bars, 5 µm.

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