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Fig. 1. Cytokines regulate the production of IB1/JIP-1. Western-blot analysis of INS-1 cellular extracts revealed that the levels of IB1/JIP-1 decreased by 80% in INS-1 cells and in freshly isolated rat islets treated with cytokines [IL-1ß (2x105 units µg–1) TNF-{alpha} (105 units µg–1) and IFN-{gamma} (10 ng ml–1)] during a period of 24 hours compared with controls (Ctrl). All lanes were loaded with 50 µg total proteins and normalized with tubulin (Tub).





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