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Fig. 2. Regulated production of IB1/JIP-1 by gene transfer approach. The IB1/JIP-1 content was measured from INS-1 cells infected with different adenovirus constructs. Western-blot analysis and quantitative assessment of these data showed that IB1/JIP-1 content is increased by 300% in cells overproducing IB1/JIP-1 (ad-sIB1), whereas decreased production was observed in cells infected with the Ad-asIB1 compared with controls (Ad-GFP or Ctrl). All lanes were loaded with 50 µg total proteins.





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