Fig. 1. Corneal fibroblasts transfected to express GFP-zyxin were plated on glass dishes and labeled for (A) vinculin and (B) F-actin organization. (A) GFP-zyxin (green) and vinculin (red) were co-localized into punctate spots (yellow) in the lamellipodia, as well as larger, more elongated structures along the cell body. (B) Yellow spots demonstrate that GFP-zyxin (green) is localized to the ends of the F-actin stress fibers (red), which is the expected localization of focal adhesions. Weaker GFP-zyxin labeling in a periodic pattern along the stress fibers is also observed. Bar, 20 µm.

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