Fig. 1. arx-2(RNAi), arx-5(RNAi) and wsp-1(RNAi) show defects in morphogenesis. (A-D) Time-lapse differential interference contrast (DIC) images of wild-type (A), arx-2(RNAi) (B), arx-5(RNAi) (C) and wsp-1(RNAi) (D) embryos. Images were obtained at 280, 360, 420 and 500 minutes after the first cleavage. Representative hypodermal cells are indicated with arrowheads. In wild-type embryos, hypodermal cells migrated toward the ventral midline between 280 and 360 minutes. By 420 minutes, the embryo had rotated 90° and started elongation (A). These processes were not observed in RNAi-treated embryos (B-D). (E-H) Localization of adherens junction marker AJM-1 fused with GFP is shown for wild-type embryos at approximately 420 minutes (E). The terminal phenotype of arx-2(RNAi) (F), arx-5(RNAi) (G) and wsp-1(RNAi) (H) embryos. An arrowhead indicates a hypodermal cell, and an arrow indicates the pharynx. All embryos are shown with the anterior positioned to the left. The wild-type embryos in A at approximately 420 and 500 minutes and in E are lateral views; all other images are ventral views. Bar in H, 10 µm.

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