Fig. 2. arx-2(RNAi) and wsp-1(RNAi) embryos show defects in hypodermal cell migration. (A-C) Ventral view of time-lapse recording of hypodermal cell marker AJM-1 fused with GFP in wild-type (A), arx-2(RNAi) (B) and wsp-1(RNAi) (C) embryos. The 3D images of AJM-1 localization were obtained at approximately 340, 360, 380, 400 and 430 minutes after the first cleavage. Some of the contralateral pairs of hypodermal cells are numbered. The pharynx is indicated with arrows. (D,E) Dorsal view of AJM-1 expression in wild-type (D) and arx-2(RNAi) (E) embryos. Treatment by arx-2(RNAi), which is lethal, showed normal AJM-1 expression on the dorsal side, although the width of the dorsal cells was narrowed due to the inability of the ventral cells to close (marked by asterisk). Bar, 10 µm.

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