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Fig. 2. Detection of RhoA in membrane fractions from CD8 cells. (A) Fractions
enriched in plasma membrane (low speed pellet, LS) or in intracellular
vesicles (high speed pellet, HS) were prepared from CD8 cells in basal
condition and after forskolin stimulation. Equal amount of protein (30
µg/lane) were subjected to western blotting analysis (see above) with
anti-RhoA-specific antibody (1:500) and revealed with
alkaline-phosphatase-conjugated anti-mouse IgG (1:5000). (B) Densitometric
analysis (mean values±s.e., n=4) of the RhoA 22 kDa band.
*P<0.001.
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