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Fig. 3. (A) Detection of Rho-GDI in membrane fractions from CD8 cells. Membrane
fractions enriched in plasma membrane (LS) or in intracellular vesicles (HS)
were prepared from control and forskolin-stimulated CD8 cells. Equal amounts
of protein (30 µg/lane) were separated by gel electrophoresis and
immunoblotted with anti-Rho-GDI antibody (1:500). Results are representative
of three independent experiments. (B) Densitometric profile (mean
values±s.e., n=3) of the 29 kDa band.
*P<0.001.
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