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Fig. 1. Zinc finger two is required for nuclear targeting. (A) Epitope tagged mouse WT1 constructs. Act, activation domain; Dim, dimerisation domain; Rep, repression domain; RRM, putative RNA recognition motif; T7, tag; ZF, zinc finger. Insertions due to alternative splicing: `17 aa' and `KTS'. (B) Constructs were transiently transfected into Cos7 cells. DAPI stain (left) and corresponding immunofluorescence of tagged protein (right) are shown.





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