Fig. 5. Nuclear import and localization of co-expressed isoforms: T7-tagged WT1 (+KTS) and Myc-tagged WT1 (–KTS) at 30 hours after plasmid injection. Western blot (A) showing the expression of both full-length constructs, and (B) of full-length Myc-tagged WT1 (–KTS) and T7-tagged CT (C-terminus +KTS). The slower migration of WT1(–KTS) is due to the larger size of the Myc versus the T7 tag. Co-expression of full-length isoforms results in the absence of signal in snurposomes: FITC image detecting the T7 tag (C), corresponding DAPI (D) and phase-contrast (E) images, highlighting Cajal bodies (arrows in E). In contrast, co-expression of only the C-terminus of the +KTS isoform, together with full-length –KTS, results in immunostaining of snurposomes: FITC image detecting the T7-tag (F), corresponding DAPI (G) and phase-contrast (H) images, highlighting B-snurposomes and Cajal bodies (arrows in F and H). Bar, 10 µm.

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