Fig. 8. Stability of interaction of WT1, CT and CTF1 with the nascent RNA transcripts of lampbrush chromosomes. Isolated nuclei were spread and washed extensively in solutions containing 200 mM, 400 mM and 600 mM NaCl. Although the chromosomes became more condensed in the higher salt conditions, most of the anti-T7 signal on the loop matrix (A,D,G) was retained, albeit in a more compact form. The corresponding DAPI (B,E,H) and phase-contrast (C,F,I) images are shown. Bar, 10 µm. (J,K,L) Salt stability of RNP immunoprecipitated from oocytes expressing WT1, CT and CTF1 (see text for details).

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