Fig. 3. hPARP-3 preferentially localizes to the daughter centriole throughout the cell cycle. (A) Confocal imaging of the subcellular distribution of hPARP-3 (red) at the centrosome marked with the anti-p34^cdc2 antibody (green) in HeLa cells; d is a merge of a and b; (B) Colocalization of hPARP-3 immunostained with the polyclonal anti-hPARP-3 antibody 1650 (red) with HeLa HC1 cells stably expressing centrin (green) fused to GFP (Piel et al., 2000). h is a merge of e and f. (C) Merged images showing an asymmetric distribution of hPARP-3 (red) and centrin fused to GFP in S/G2 cells. hPARP-3 (red) was immunostained as in B. Strongly fluorescent dots of GFP-centrin (insets m and j) are attributed to the mother centrioles. (D) Immunofluorescence microscopy analysis using the 1650 polyclonal anti-hPARP-3 antibody (red) and the anti-acetylated -tubulin monoclonal antibody (green) showing the primary cilium of the mother centriole. (E) hPARP-3 subcellular distribution throughout the cell cycle. Colocalization of hPARP-3 with GFP-centrin in HeLa HC1 cells by confocal microscopy analysis using the polyclonal anti-hPARP-3 antibody 1650 (red). For all pictures, the magnifications are details of the area surrounding the arrowheads. Nuclei in c, g, i, p and s are stained with Hoechst or with DAPI in S phase. Bars, 10 µm.

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