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Fig. 4. hPARP-3 is detected in purified centrosomes. (A) Immunostaining of centrosome preparations from KE 37 cells. Immunolabelling was carried out using an anti-p34cdc2 antibody (green) and the polyclonal anti-hPARP-3 antibody 1650 (red). Bar, 2 µm. (B) Western blot of purified recombinant hPARP-3 (lane a), a sample of purified centrosomes (108) (lane b) and a KE 37 lysate containing a highly enriched centrosome preparation present in both the Triton-insoluble (lane c) and Triton-soluble fractions (lane d).





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