Fig. 4. Immunolocalization of RyR1 in cultured skeletal muscle cells. Double immunofluorescence analysis of primary cultures prepared from E9.5 somites (somitic cells, A-C), E11 hind limbs (embryonic myoblasts, D-F), E16 hind limbs (fetal myoblasts, G-I) and 2-week-old mice (satellite cells, L-N). After differentiation (72 hours), the cells were fixed and reacted with an anti-RyR1-specific anti-serum (A,D,G,L) together with the anti-MyHC antibody MF20 (B,E,H,M). Phase contrast micrographs of each field are also shown (C,F,I,N). Bar, 10 µm.

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