Fig. 1. Complementary muscle grafts with GFP-labelled host, 0 to GFP (A,C,D) and GFP labelled donor, GFP to 0 (B). (A,B) Macroscopic appearance under UV light of grafts of tibialis anterior (TA) muscle to the TA bed. (A) 49 days p.o., (B) 70 days p.o. Bar in B, 2 mm. (C,D) Complementary labelling in a 0/nLacZ to GFP/0 graft, 50 days p.o. Cross-section at graft(g)/host(h) border. (C) X-gal plus eosin staining, (D) GFP (green) and Hoechst (blue) nuclear fluorescence. The donor was homozygous for the nLacZ knock-in (and for DesKO). There are several GFP-positive muscle fibres that have nLacZ-positive nuclei (arrows), indicating co-fusion of donor myoblasts with host muscle fibres. Bar in C, 100 µm.

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