Fig. 2. Unlabelled graft to a double-labelled host, 0/0 to GFP/nLacZ, 77 days p.o. (A-D), and nLacZ-labelled graft to a GFP host (complementary label), 0/nLacZ to GFP/0, 75 days p.o. (E,F). Migration of host cells into the graft. The same region of the host(h)/graft(g) border in adjacent sections is shown in fluorescence (A,C,E) and after Xgal/eosin staining (B,D,F). (A,B,E,F) Cross-sections of proximal parts, (C,D) longitudinal sections of distal parts of the graft site. (A,E) Within the graft there are numerous fibers with host label (GFP) that can be followed through serial sections, as indicated by green contours in B,F. In B,D, a few nLacZ-positive nuclei are found in GFP-positive fibres close to the host (arrows). In F, nearly all of the GFP-positive fibres show blue nuclei. C,D,F indicate immigration of host stem cells and co-fusion with graft muscle fibres rather than ingrowth of host muscle fibres. In C, a green margin along bent muscle fibres is due to a local GFP overspill during thawing of the section (Jockusch et al., 2003). Bar, 100 µm.

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