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Fig. 4. VCX1 plays an important role in Ca2+ homeostasis in the pmc1{Delta} and pmr1{Delta}/pmc1{Delta} strains. (A) Total cellular Ca2+ levels measured with flame photometry. (B) Cytosolic free Ca2+ levels measured with an aequorin Ca2+ reporter system. The arrow represents the addition of 100 mM CaCl2 (see Materials and Methods for further details).





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