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PARP-3: a marker for daughter centrioles

Poly (ADP-ribose) polymerases (PARPs) are enzymes that add ADP-ribose moieties to specific acceptor proteins. Best characterized are PARP-1 and PARP-2, which function as survival factors that modify nuclear proteins such as histones and topoisomerases during DNA damage signalling. Gilbert de Murcia and co-workers have now cloned and characterized a novel member of the PARP family, PARP-3, which might have a rather different function (see p. 1551). PARP-3 displays PARP activity in vitro, and its catalytic domain shares significant sequence similarity (61%) with PARP-1. The authors find that the protein localizes to centrosomes, where it associates with PARP-1. In contrast to PARP-1, however, PARP-3 is present only at daughter centrioles – and thus represents the first known marker protein for these structures. Murcia and co-workers show that overexpression of PARP-3 or its noncatalytic N-terminal domain causes cells to arrest at the G1/S transition of the cell cycle. This finding is particularly significant given recent work suggesting that centrosomes play a critical role in cell cycle progression. Moreover, since PARP-1 is also implicated, the enzymes might link DNA damage surveillance to a checkpoint controlling mitotic fidelity.

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PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression

Angélique Augustin, Catherine Spenlehauer, Hélène Dumond, Josiane Ménissier-de Murcia, Matthieu Piel, Anne-Catherine Schmit, Françoise Apiou, Jean-Luc Vonesch, Michael Kock, Michel Bornens, and Gilbert de Murcia
JCS 2003 116: 1551-1562. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?