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Fig. 1. Specificity of antibodies to Eg7G, XCAP-E1, TopoII and ß-tubulin in
XL2 cell extract. Proteins in X. laevis XL2 cell lysate
(2x105 cells) were separated onto 7% SDS-polyacrylamide gel,
transferred onto nitrocellulose membranes and immunodetected with mixture of
purified Eg7G and monoclonal antibodies against ß-tubulin (lane1) or
XCAP-E1 antibodies (lane 2) or monoclonal anti-topoII antibodies (lane 3).
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