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Fig. 7. Immunolocalization of XCAP-E and B23 in control cells and after actinomycin
treatment. XL2 cells were incubated for 6 hours in the medium with 5 µg/ml
actinomycin D (E-H) or without the drug (A-D). After fixation, cells were
processed for immunofluorescence staining with polyclonal anti-XCAP-E1 (B,F)
and monoclonal anti-B23 (C,G) antibodies. Cells were stained for DNA
visualization with DAPI (A,E). Triple DAPI/XCAP-E/B23 labeling is shown (D,H).
Bar, 5 µm.