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Fig. 9. Immunolocalization of XCAP-E and topoII on the nucleolus in control cells
and after actinomycin treatment. XL2 cells were incubated for 6 hours in the
medium with 5 µg/ml actinomycin D (E-H) or without the drug (A-D). After
fixation, cells were processed for immunofluorescence staining with polyclonal
anti-XCAP-E1 (C,G) and monoclonal anti-topoII (D,H) antibodies. Cells were
observed by phase contrast microscopy (A,E) and stained for DNA visualization
with DAPI (B,F). Bar, 5 µm.
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