Movies 1 and 2. Eng1p is involved in cell separation in Schizosaccharomyces pombe. Cell growth of eng1 disruptants was monitored using time-lapse differential interference contrast (DIC) microscopy. In wild-type cells, ce3ll division produced two equivalent cells that separate immediately after cell division (Movie 1). In contrast, eng1 mutants have a defect in completion of septum dissolution, resulting in the formation of groups of four cells (Movie 2).

Movies 3 and 4. Three-dimensional reconstruction of eng1p localization. Cells containing the eng1-GFP fusion allele were observed under a confocal microscope, z-sections of 0.3 mm were taken and the image was reconstructed using the LSM510 software. The eng1-encoded b-glucanase localizes to a ring-like structure that completely surrounds the septum region during cell separation (Movies 3 and 4).