Fig. 1. eng1p is an endo-ß-1,3-glucanase. (A) Schematic representation of the S. cerevisiae (ScEng1p) and S. pombe endo-ß-1,3-glucanases (Speng1p). The structure of each protein is shown at the same scale (indicated at the top as the number of amino acids), with a gray rectangle indicating the conserved region between the proteins. A black box in the N-terminal region indicates the predicted secretory signal sequence, while triangles mark the position of putative N-glycosylation sites. White boxes represent Ser/Thr-rich regions (indicated by S/T) or the Thr-rich domain (marked with a T). (B) ß-glucanase activity against laminarin (ß-1,3-glucan) in cells (strain h20) transformed with plasmid pAB10 (carrying P_nmt1-eng1) or vector alone (pREP3X). Cells were grown for 16 hours in the presence (white bars) or absence (black bars) of thiamine to induce the expression of the eng1⁺ gene. (C) Enzymatic activity of cells overexpressing eng1⁺ against pustulan (ß-1,6-glucan) or p-nitrophenyl-ß-D-glucoside (PNPG).

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