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Fig. 6. eng1p localizes to the septum. (A) Wild-type cells containing the
eng1-GFP fusion allele were grown to early-log phase and stained with
Calcofluor before microscopic observation. Calcofluor fluorescence (CF), GFP
fluorescence and the overlay of both images (Merge) are shown. (B)
Three-dimensional reconstruction of eng1p localization. Cells containing the
eng1-GFP fusion allele were observed under a confocal microscope and
z-sections of 0.3 µm were taken. The image was reconstructed using LSM510
software.
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