Fig. 7. eng1⁺ expression and ß-1,3-glucanase activity vary periodically during the cell cycle. Synchrony was induced by arrest-release of cdc25-22 mutants and samples were taken at the indicated time points (minutes) for RNA extraction (A) or ß-glucanase activity determinations (B). (A) RNA was probed with specific probes for eng1⁺ or ura4⁺. The graph represents the anaphase index () or septation index (•) at each time point. In this experiment, the peak of septum formation occurred at 70-90 minutes. (B) ß-glucanase activity was assayed using laminarin as substrate in samples from a cdc25-22 (upper panel) or cdc25-22 eng1 (lower panel) mutant strains. The septation index for each strain is indicated (•). The anaphase index was determined by counting the percentage of anaphase cells (cells with two nuclei and without a septum) after DAPI staining. The septation index was determined by counting the percentage of cells with septum after calcofluor staining.

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