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Fig. 8. ace2+ is required for cell separation. (A) Expression
of eng1+ and act1+ genes in wild-type
(lane 1), ace2-null mutants (cells lacking the SPAC6G10.12C ORF, lane
2), or cells containing a deletion of SPAC4G8.13c (lane 3). (B) Morphology of
the mutant cells grown in rich medium to early-log phase. Wild-type (1) and
mutants lacking SPAC4G8.13c (2) show a normal morphology, while ace2
mutants (3) have a clear mycelial and branched phenotype, indicating severe
cell separation defects.
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