Fig. 7. Localisation of proteasomes in relation to c-Myc in proteasome inhibitor treated cells. (A) The distribution of c-Myc and proteasomes in a proteasome-inhibitor-treated cell is shown at high resolution by the confocal images in a and b, respectively. The colocalisation of c-Myc and proteasomes is shown in c-e. In c the confocal images in a and b are overlayed. The relative fluorescence intensity from c-Myc (green) and proteasomes (red), along the white line in c is quantified in d. (e) Reconstitution of the stack of confocal xy plane images; e' and e'' show yz and xz sections through the stack of images, respectively. The positions of the sections are shown by the fine white lines in e. Bar, 10 µm. (B) The distribution of c-Myc-GFP and proteasomes in cells expressing increased levels of c-Myc in the absence of proteasome inhibitor is shown by the confocal images a-d. Proteasomes accumulate specifically in the nucleoli that contain c-Myc (e-g, yellow arrows). Nucleoli lacking c-Myc remain devoid of nucleolar proteasome staining (e-g, white arrowheads)

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