Fig. 3. Rank order profiles of normalized cDNA expression by the colonies characterized in Fig. 2. Phosphorimage signal intensities for each probe were quantified and standardized against total cDNA. Immature osteoprogenitor colonies identified by replica plating that did not express detectable levels of ALP were classed as immature or primitive osteoprogenitors (OP) compared to more mature osteoprogenitors/preosteoblasts (Pre-OB) that did express low levels of ALP message; OBs and Mature OBs are defined as in Fig. 2. All colonies were rank-ordered according to the paradigm outlined in the Results.

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