Fig. 1. GFP containing a PTS1 motif is imported into peroxisomes. Wild-type nematodes were injected with the construct p_HSP16/2GFP—SKL to create the line, Ex[p_HSP-16/2GFP—SKL;pRF4(rol-6su1006)]. Transgenic animals were heat-shocked for 4 hours at 35°C and recovered at 20°C for 2 hours before photography. A distinctly punctate pattern was observed in intestinal cells (A), in hypodermal cells (B) and in developing embryos (C). Note that for photography we selected genetically mosaic animals in which only a few cells harbored the transgene.

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