Fig. 2. dsRNA interference directed against the C. elegans PEX-5 homolog C36C6.6, prx-5, results in larval arrest. Young adult hermaphrodites injected with dsRNA targeted to exon 6 of prx-5 laid eggs for a 12 hour period, 16 hours after injection. (A) RNAi knockdown of prx-5 results in developmental arrest at the L1/L2 stage. (B) Identical magnification of a prx-5(RNAi) nematode 3 days after being laid, and a time-matched wild-type worm. Bar, 10 µm. (C) After a 16 hour recovery period postinjection, ten viable injected worms were placed on a plate for 12 hours to measure egg production. Egg production is reduced in prx-5(RNAi) treated animals as compared to an unc-22(RNAi) control injection.

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