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Fig. 4. Quantification of soluble VEGF isoforms. Soluble VEGF isoforms (VEGF 120
and VEGF 164) were determined in conditioned medium after either 22 hours
hypoxia or normoxia. Bars represent the means ± standard deviations
(triplicates from three independent experiments, normalized to protein
concentrations). Statistical differences were calculated as: wild-type
normoxia vs HIF-1-null normoxia; wild-type hypoxia vs HIF-1-null hypoxia;
wild-type normoxia vs wild-type hypoxia. **P
0.01.
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