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Fig. 7. Effect of chemical glycolysis inhibition on type-II collagen expression.
Wild-type epiphyseal chondrocytes were incubated with or without 0.2 M
2-deoxyglucose, cultured under normoxic or hypoxic conditions for 22 hours,
and type-II collagen gene expression was analysed by quantitative real-time
PCR. Bars represent -fold changes of mRNA levels compared with untreated
values (normoxic expression taken as 1.0). Data are given as mean ±
standard deviations (triplicates from three independent experiments).
**P
0.01.
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