Fig. 6. Developmental potential of BMSSCs clones in vivo. Immunoselected STRO-1^BRIGHT/VCAM-1⁺ marrow-derived clones were expanded in vitro, then implanted subcutaneously into SCID mice using HA/TCP carrier. Implants were harvested 8 weeks after the transplant. (A) New bone formation containing osteocytes (arrow) was observed for a proportion of clones developing at the HA/TCP particle surfaces together with surrounding fibrous and hematopoietic tissue (BM). The sections were counter stained with haematoxylin and eosin (200x). (B) The osteocytes and bone lining cells (arrow) were found to be of human origin, as demonstrated by in situ hybridization using a DNA probe specific to the human alu repeat sequence (200x).

Return to article