Fig. 1. Cloning of TAF7L and characterisation of anti-TAF7L antibodies. (A) Alignment of the sequences of TAF7L from human and mouse with those of TAF7 from mouse, Drosophila melanogaster and yeast (Saccharomyces cerevisiae). Highly conserved amino acids are shown in white on a black background. Positions conserved in at least three of the proteins are boxed in grey. Amino acids were classified as follows. Small residues: P, A, G, S, T; hydrophobic: L, I, V, A, F, M, C, Y, W; polar/acidic: D, E, Q, N; basic: R, K, H. The peptide used to generate the antibodies is underlined. (B) Cos cells were transfected with a TAF7L (lane 2) or empty (lane 1) expression vector and the recombinant TAF7L detected with monoclonal antibody 46TA. TAF7L is also visible in the testis nuclear extract (lane 3). (C) TAF7L, TBP, TAF12 and TAF13 were detected in total extracts from the tissues shown above each lane.

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