Fig. 7. Two hybrid interactions. (A) The TAF7L bait and the prey cDNA library are shown schematically. AD, activation domain; DBD, DNA binding domain. Graphical representation of quantitative two hybrid ß-galactosidase assays. The GAL4-DBD TAF7L chimera shown above the graph was tested with the GAL4-AD chimeras shown below each column. The results obtained with the two independent TAF1(1070-1226) clones are shown. — indicates negative controls with the GAL4-AD alone. The values represent the average of two independent experiments. (B) Schematic representation of TAF1 and the isolated clones. TAF1 is shown along with the histone acetyl-transferase (HAT) and RAPiD domains. A comparison of the TAF1 RAPiD domain and the region found in the two hybrid clones is also depicted. The numbers above represent the TAF1 amino acids at the N and C-termini of the domains.

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